Cryotec Methods

The Cryotec Method The Safest and Most Effective Cryopreservation Method in the World with a 100% survival rate.

The Cryotec Method is a vitrification system used to safely cryopreserve and thaw oocytes and embryos at any stage. This method allows us to achieve 100% survival after thawing, which is one of the best indicators of the safety of cryopreservation. REPROLIFE began to spread this method globally in 2012, and it has been applied to hundreds of thousands of clinical cases in over 70 countries. In each country, the Cryotec Method has been able to significantly improve the clinical data of IVF centers and facilities that had previously been using the “slow freezing” method and other conventional systems of vitrification.
The purpose of cryopreservation is to safely preserve oocytes and embryos, which hold a life within, without hurting them until the day they are thawed and implanted into the mother’s body. The Cryotec Method vitrification technique enables us to achieve this goal in the most efficient and reliable way yet invented

Features of the Cryotec Method

  • Consistent 100% survival of normal blastocysts, embryos, and oocytes when the protocol is properly followed. Our officially certified trainers instruct each user until they master the protocol to duplicate 100% survival rates.
  • The unequaled survival rate also represents minimal damage to oocytes or embryos, if any, meaning the fertility potential of each oocyte or embryo is not adversely affected by the process of cryopreservation.
  • The simplest and easiest-to-learn protocol for anyone to yield repeatable survival results.
  • Even effective to most oocytes and embryos in bad conditions, such as eggs of patient with hig age.

Modifications Made to the Conventional Method

Dr. Kuwayama, inventor of a number of conventional vitrification methods, identified the weaknesses of the most popular imperfect vitrification method, which was the latest at the time, with about 90% survival rates. He then made 18 modifications to it in order to overcome all of the weaknesses of his previous technique, which were also the main causes of egg loss: Thus, the Cryotec Method was born. Safest and most effective in the industry, the Cryotec Method enabled anyone to achieve the best possible outcome without mistake, regardless of their experience or knowledge of vitrification.


  • Most efficient formulas to ensure successful vitrification and thawing.
  • All solutions are serum-free and protein-free with an ultra-long shelf life of 12 months.
  • These solutions require no refrigeration during shipping.
  • Endotoxin-free trehalose in these solutions functions as a great substitute for sucrose with greater safety and higher viscosity.


The Vitri Plate enables easier and safer vitrification with an ergonomic design: The circular wells are ideal preventing osmotic shock.
  • The Cryotec, the cryopreservation carrier device, can be securely placed in the groove of the Vitri Plate.
  • The Warm Plate is also highly ergonomic: The sloped TS well prevents the formation of bubbles during thawing without needing a separate TS dish.
  • Like the Vitri Plate, the wells of the Warm Plate have a spherical bottom, which allows solutions to gradually mix with each other. Hundreds of points of our experimental data show this greatly prevents osmotic shock.


  • One protocol is applicable to oocytes, embryos, and blastocysts with a diameter of 220μm or less. (Larger blastocysts require a pre-shrinking process)
  • The completion of equilibration within each solution is identified by the observation of objective facts.
  • The Cryotec Method trainers strongly advise you against adjusting VS droplets on the carrier device during the loading process: Surface tension disruptions could deform and fatally damage oocytes and embryos during vitrification. Instead, they train users to consistently make properly sized droplets.


Medical Surgical Articles & Requisites Trading
Al Barsha 2, St.329-42B_villa 43. P.O. Box. 6525,
Dubai, United Arab Emirates.
+971 557 365992 +971 506 228980
+971 4 452 6865